Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights
نویسندگان
چکیده
منابع مشابه
Coxsackievirus B3 protease 3C induces cell death in eukaryotic cells
Abstract: Coxsackievirus B3 (CVB3) is the most common agent known to cause viral myocarditis. The viral genome encodes a single polyprotein that is cleaved to produce several proteins by virally encoded proteases. Most of this proteolytic processing is catalyzed by a cysteine protease called 3C. The 3C protease plays major role in viral replication and cellular damage. To understand the mecha...
متن کاملcoxsackievirus b3 protease 3c induces cell death in eukaryotic cells
background and aims: coxsackievirus b3 (cvb3) is the most common agent known to cause viral myocarditis. the viral genome encodes a single polyprotein that is cleaved to produce several proteins by virally encoded proteases. most of this proteolytic processing is catalyzed by a cysteine protease called 3c. the 3c protease plays major role in viral replication and cellular damage. methods: to un...
متن کاملAntiviral activity of coxsackievirus B3 3C protease inhibitor in experimental murine myocarditis.
BACKGROUND We investigated the efficacy of a 3C protease inhibitor (3CPI) in a murine coxsackievirus B3 (CVB3) myocarditis model. CVB3 is a primary cause of viral myocarditis. The CVB3 genome encodes a single polyprotein that undergoes a series of proteolytic events to produce several viral proteins. Most of this proteolysis is catalyzed by the 3C protease (3CP). METHODS AND RESULTS By way of...
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Protease 2A (2Apro) of coxsackievirus B3 (CVB3) plays a major role in viral replication. In case of infection, viral proteins are being synthesized from viral mRNA using host biosynthesis machinery. 2Apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4G. The enzyme plays an essential role in viral replic...
متن کاملEffect of T68A/N126Y mutations on the conformational and ligand binding landscape of Coxsackievirus B3 3C protease.
3C protease of Coxsackievirus B3 (CVB3) plays an essential role in the viral replication cycle, and therefore, emerged as an attractive therapeutic target for the treatment of human diseases caused by CVB3 infection. In this study, we report the first account of the molecular impact of the T68A/N126Y double mutant (Mutant(Bound)) using an integrated computational approach. Molecular dynamics si...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology Communications
سال: 2016
ISSN: 2053-230X
DOI: 10.1107/s2053230x16018513